PepMix™Human(SOX-2)

ProductCode:PM-SOX-2

Poolof77peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughTranscriptionfactorSOX-2(Swiss-ProtID:P48431)ofHomosapiens(Human)forTcellassays.
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Cancer,Microphthalmiasyndromictype3
DeliveryTime:2-5days

PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.

BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried

PepMix™Human(SOX-2)

SelectedReferences
"DysfunctionofPSA-specificCD8+TCellsinProstateCancerPatientsCorrelateswithCD38andTim-3Expression"
Jappetal.,CancerImmunolImmunother.(2015)-PMID:26289091
"AnHPV-E6/E7ImmunotherapyPlusPD-1CheckpointInhibitionResultsinTumorRegressionandReductioninPD-L1Expression"
Riceetal.,CancerGeneTherapy(2015)-PMID:26337747
"Survivin-specificCD4+TCellsareDecreasedinPatientswithSurvivin-positiveMyeloma"
Lockeetal.,JImmunotherCancer(2015)-PMID:25992291
"APhaseITrialCombiningDecitabine/dendriticCellVaccineTargetingMAGE-A1,MAGE-A3andNY-ESO-1forChildrenwithRelapsedorTherapy-RefractoryNeuroblastomaandSarcoma"
Krishnadasetal.,CancerImmunolImmunother.(2015)-PMID:26105625
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"PresenceofCirculatingHer-2-reactiveCD8+T-CellsisAssociatedwithLowerFrequenciesofMyeloid-derivedSuppressorCellsandRegulatoryTCells,andBetterSurvivalinElderlyBreastCancerPatients"
KiniBailuretal.,BreastCancerResearch(2015)-PMID:n.a.
"STAT3SignalingContributestotheHighEffectorActivitiesofInterleukin-15-derivedDendriticCells"
Okadaetal.,ImmunolCellBiol.(2015)-PMID:25582338

ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext

Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK

PepMix™Human(SOX-2)

TechnicalData:
ApplicationAntigenspecificT-cellstimulation
CancerbioMarkerresearch
Immunemonitoring
T-cellassays
T-cellexpansion
ProteinNameTranscriptionfactors/activators
OrganismHomosapiens(Human)
Indication/TopicCancer
Microphthalmiasyndromictype3
Numberofpeptides77 

FurtherInformation:
Amount1vialcontaining15nmol(appr.25µg)perpeptide
SpecificationsPeptidescan(15merswith11aaoverlap)
DocumentationProtocolandDatasheet
Swiss-ProtID
P48431
NCBINP_003097
SequenceMYNMMETELKPPGPQQTSGGGGGNSTAA
AAGGNQKNSPDRVKRPMNAFMVWSRGQR
RKMAQENPKMHNSEISKRLGAEWKLLSE
TEKRPFIDEAKRLRALHMKEHPDYKYRP
RRKTKTLMKKDKYTLPGGLLAPGGNSMA
SGVGVGAGLGAGVNQRMDSYAHMNGWSN
GSYSMMQDQLGYPQHPGLNAHGAAQMQP
MHRYDVSALQYNSMTSSQTYMNGSPTYS
MSYSQQGTPGMALGSMGSVVKSEASSSP
PVVTSSSHSRAPCQAGDLRDMISMYLPG
AEVPEPAAPSRLHMSQHYQSGPVPGTAI
NGTLPLSHM
ProteinLength(aa)317